iPhoneAlle anzeigen
iPhone 11iPhone 11 ProiPhone 11 Pro MaxiPhone 12iPhone 12 ProiPhone 12 Pro MaxiPhone 12 miniiPhone 13iPhone 13 ProiPhone 13 Pro MaxiPhone 13 miniiPhone 14iPhone 14 PlusiPhone 14 ProiPhone 14 Pro MaxiPhone 15iPhone 15 PlusiPhone 15 ProiPhone 15 Pro MaxiPhone 16iPhone 16 PlusiPhone 16 ProiPhone 16 Pro MaxiPhone 16eiPhone 8iPhone 8 PlusiPhone SE 2020iPhone SE 2022iPhone XiPhone XRiPhone XSiPhone XS Max
Galaxy A-SerieAlle anzeigen
Galaxy A12Galaxy A13Galaxy A13 5GGalaxy A14Galaxy A15Galaxy A16Galaxy A17 5GGalaxy A20eGalaxy A21Galaxy A22Galaxy A23Galaxy A24Galaxy A25Galaxy A26 5GGalaxy A32Galaxy A33Galaxy A34Galaxy A35Galaxy A36 5GGalaxy A40Galaxy A41Galaxy A42Galaxy A50Galaxy A51Galaxy A52Galaxy A53Galaxy A54Galaxy A55Galaxy A56 5GGalaxy A70Galaxy A71Galaxy A72
Redmi SeriesAlle anzeigen
Redmi 10Redmi 13CRedmi 15 5GRedmi Note 10Redmi Note 10 ProRedmi Note 11Redmi Note 11 ProRedmi Note 11 Pro Plus 5GRedmi Note 11sRedmi Note 12Redmi Note 12 5GRedmi Note 12 ProRedmi Note 12 Pro 5GRedmi Note 12 Pro PlusRedmi Note 13Redmi Note 13 5GRedmi Note 13 ProRedmi Note 13 Pro PlusRedmi Note 14Redmi Note 14 Pro PlusRedmi Note 8Redmi Note 8 ProRedmi Note 9Redmi Note 9 Pro
- Startseite
Bücher Englische Bücher Wissen & Bildung Naturwissenschaft, Medizin, Informatik, Technik Physik & Astronomie Multi-Parameter Protein Analysis with an Electro-Switchable Biosurface
Derzeit nicht verfügbar
Handgeprüfte Gebrauchtware
Bis zu 50 % günstiger als neu
Der Umwelt zuliebe
Multi-Parameter Protein Analysis with an Electro-Switchable Biosurface
Andreas Langer (Taschenbuch, Englisch)
★★★★★
☆☆☆☆☆ Keine Bewertungen vorhanden
Optischer Zustand
Beschreibung
Measurements in stationary or mobile phases are fundamental principles in protein analysis. Although the immobilization of molecules on solid supports allows for the parallel analysis of interactions, properties like size or shape are usually inferred from the molecular mobility under the influence of external forces. However, as these principles are mutually exclusive, a comprehensive… characterization of proteins usually involves a multi‐step workflow. In this dissertation, I show how these measurement modalities can be reconciled by tethering proteins to a surface via dynamically actuated nanolevers. Short DNA strands, which are switched by alternating electric fields, are employed as capture probes to bind target proteins. By swaying the proteins over nanometer amplitudes and comparing their motional dynamics to a theoretical model, the protein diameter can be quantified with Angstrom accuracy. Alterations in the tertiary protein structure (folding) and conformational changes are readily detected.
The general applicability of electrically switchable DNA layers for the efficient high‐ throughput screening of a large number of low molecular weight compounds is demonstrated at the example of the model enzyme human dihydrofolate reductase. An affinity ranking of several small molecules over a broad dynamic range is performed via a competitive assay and supplementary to the use as a bare screening tool, conformational changes between the binary and the ternary enzyme complex are uncovered.
Finally, the presented chip‐based method is used to study DNA polymerases and their interaction with nucleic acids. The association and dissociation of DNA polymerases is monitored in real‐time by analyzing changes in the DNA motion that occur due to polymerase binding. Chemical rate constants of association and dissociation, affinity constants and thermodynamic parameters are determined. The enzymatic activity is evaluated and even exonuclease activity can be observed. Simultaneously, information on the size of the DNA‐ polymerase complex is obtained and conformational changes in the ternary polymerase‐dNTP‐ DNA complex are revealed (match/mismatch situation). Dieses Produkt haben wir gerade leider nicht auf Lager.
Handgeprüfte Gebrauchtware
Bis zu 50 % günstiger als neu
Der Umwelt zuliebe
Technische Daten
Erscheinungsdatum
01.12.2015
Sprache
Englisch
EAN
9783941650947
Herausgeber
Verein zur Förderung des Walter Schottky Instituts der Technischen Universität München
Sonderedition
Nein
Autor
Andreas Langer
Seitenanzahl
106
Einbandart
Taschenbuch
Schlagwörter
molecular dynamics, interaction analysis, biomolecules
Thema-Inhalt
P - Mathematik und Naturwissenschaften
-.-
★★★★★
☆☆☆☆☆ Leider noch keine Bewertungen
Leider noch keine Bewertungen
Sicher bei rebuy kaufen
Schreib die erste Bewertung für dieses Produkt!
Wenn du eine Bewertung für dieses Produkt schreibst, hilfst du allen Kund:innen, die noch überlegen, ob sie das Produkt kaufen wollen. Vielen Dank, dass du mitmachst!
Sicher bei rebuy kaufen