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Bücher Wissen & Bildung Naturwissenschaft, Medizin, Informatik, Technik Chemie Anorganische Chemie NMR Sensitivity Enhancement of functional Biomolecules: Design and Development of PHIP-based Hyperpolarization Approaches
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NMR Sensitivity Enhancement of functional Biomolecules: Design and Development of PHIP-based Hyperpolarization Approaches
Grit Sauer (Taschenbuch, Deutsch)
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Beschreibung
Para-Hydrogen Induced Polarization (PHIP) is one of the most efficient methods enabling enhanced sensitivity in nuclear magnetic resonance spectroscopy (NMR) and magnetic resonance imaging (MRI). So far, this hyperpolarization method has been successfully applied to only a surprisingly small number of biologically relevant molecules, all possessing rather simple structures. This outcome can be… explained taking into consideration that the molecule to be examined requires an unsaturated moiety which can undergo catalytic hydrogenation with para-enriched hydrogen that must be transferred pairwise, and added within the lifetime of the hyperpolarized state. Only when this is the case, the increase in sensitivity could be achieved.
This study was aimed on further development of PHIP technology towards its application to rather sophisticated biomolecules such as peptides and proteins, or usage for kinetic studies.
One focus of the present research was the development of a viable synthetic approach towards decoration of desired peptidic compounds with unsaturated moieties required for PHIP. To that end, non-natural amino acids containing a hydrogenizable, unsaturated group in their side chains should be incorporated into the peptides of choice. For this purpose unsaturated precursors, the so-called PHIP labels, have to be designed considering their compatibility with the solid phase peptide synthesis (SPPS). Dieses Produkt haben wir gerade leider nicht auf Lager.
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Technische Daten
Erscheinungsdatum
01.08.2016
Sprache
Deutsch
EAN
9783863877408
Herausgeber
Mensch & Buch
Sonderedition
Nein
Autor
Grit Sauer
Seitenanzahl
184
Auflage
1
Einbandart
Taschenbuch
Einbandart Details
Laminiert
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