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PCR Detection of Microbial Pathogens

(Gebundene Ausgabe, Englisch)

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Beschreibung
The polymerase chain reaction (PCR) has become an increasingly important tool in microbial diagnosis in recent years, mainly because of its rapidity, high sensitivity, and specificity. In a number of instances, parti- larly with noncultivatable and slow-growing microorganisms, reliable iden- fication and differentiation became possible only after the introduction of PCR-based methods. Because the number of published procedures is large and steadily rising, there is a need for critical evaluation, comparison of performance, and even- ally also standardization of methods to enable laboratory diagnosticians to select the optimal methodology. PCR Detection of Microbial Pathogens is designed to provide an ov- view of the possibilities and problems connected with the use of PCR-based identification and detection of important bacterial and other microbial pat- gens, among them several zoonotic agents. It is intended to provide micro- ologists and biochemists the opportunity to extend their knowledge of state-of-the-art detection procedures, as well as pre-PCR sample processing and various general aspects of PCR. The chapters of this volume have been written by investigators well conversant with molecular detection and iden- fication of microorganisms. They have contributed their extensive practical experience to the protocols, not only concerning PCR methodology itself, but also the highly important area of pre-amplification sample processing.
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Technische Daten


Erscheinungsdatum
14.11.2002
Sprache
Englisch
EAN
9781588290496
Herausgeber
Humana Press
Serien- oder Bandtitel
Methods in Molecular Biology
Sonderedition
Nein
Seitenanzahl
336
Auflage
1
Einbandart
Gebundene Ausgabe
Bandzählung
216
Thema-Inhalt
PSG - Mikrobiologie (nicht-medizinisch)
Inhaltsverzeichnis
Part I. Reviews Specificity and Performance of Diagnostic PCR Assays Konrad Sachse Pre-PCR Processing of Samples Peter Rådström, Rickard Knutsson, Petra Wolffs, Maria Dahlenborg, and Charlotta Löfström Critical Aspects of Standardization of PCR Jeffrey Hoorfar and Nigel Cook PCR Technology and Applications to Zoonotic Food-Borne Bacterial Pathogens Peter Stephensen Lübeck and Jeffrey Hoorfar Part II. Protocols Detection, Identification, and Subtyping of Actinobacillus pleuropneumoniae Joachim Frey Identification and Differentiation of Brucella abortus Field and Vaccine Strains by BaSS-PCR Darla R. Ewalt and Betsy J. Bricker Isolation of Campylobacter and Identification by PCR Mark D. Englen, Scott R. Ladely, and Paula J. Fedorka-Cray Detection and Differentiation of Chlamydiae by Nested PCR Konrad Sachse and Helmut Hotzel Detection of Toxigenic Clostridia Michel R. Popoff PCR-Based Detection of Coxiella burnetii from Clinical Samples Mustapha Berri, Nathalie Arricau-Bouvery, and Annie Rodolakis Detection and Subtyping of Shiga Toxin-Producing Escherichia coli (STEC) Peter Gallien Detection of Listeria monocytogenes Using a PCR/DNA Probe Assay Louise O'Connor Detection of Leptospira interrogans John W. Lester and Rance B. LeFebvre Detection of Pathogenic Mycobacteria of Veterinary Importance Robin A. Skuce, M. Siobhan Hughes, Malcolm J. Taylor, and Sydney D. Neill Multiplex PCR of Avian Pathogenic Mycoplasmas Mazhar I. Khan Detection and Differentiation of Ruminant Mycoplasmas Helmut Hotzel, Joachim Frey, John Bashiruddin, and Konrad Sachse Detection of Mycoplasma hyopneumoniae from Clinical Samples and Air Marylène Kobisch and Joachim Frey PCR-Detection of Hemophilus paragallinarum, Hemophilus somnus, Mannheimia (Pasteurella) hemolytica, Mannheimia spp., Pasteurella trehalosi, and Pasteurella multocida Henrik Christensen, Magne Bisgaard, Jesper Larsen, and John Elmerdahl Olsen Detection of Salmonella spp. Burkhard Malorny and Reiner Helmuth Detection of Toxoplasma gondii Jonathan M. Wastling and Jens G. Mattsson PCR-Derived Methods for the Identification of Trichinella Parasites from Animal and Human Samples Edoardo Pozio and Giuseppe La Rosa Detection of Pathogenic Yersinia enterocolitica by a Swab Enrichment PCR Procedure Rickard Knutsson and Peter Rådström Index
Höhe
229 mm
Breite
15.2 cm

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