Handbook of RNA Biochemistry

26.01.2005

Englisch

9783527308262

Wiley-VCH

Nein

931

1

Gebundene Ausgabe

Roland K. Hartmann is Professor of Pharmaceutical Chemistry at the Philipps-Universität Marburg (Germany). He studied Biochemistry at the Freie Universität Berlin where in 1988 he received the Ernst Reuter award for outstanding Ph.D. theses. His research interests include natural ribozymes, particularly ribonuclease P, interactions of RNAs with proteins and small ligands, aptamers, antisense and RNA interference. Albrecht Bindereif is Professor of Biochemistry at the University of Giessen (Germany) since 1999. He studied Biochemistry in Tübingen and at the University of California, Berkeley. His postdoctoral work was done in Michael Green's laboratory at Harvard University. His research interests focus on the mechanism and regulation of mRNA splicing, both in the mammalian system and in trypanosomes. Astrid Schön received her PhD from the Institute of Biochemistry at the University of Würzburg, and was a graduate fellow and postdoctoral associate with Dieter Söll at Yale University. She is currently a lecturer at the Institute of Biochemistry, University of Leipzig (Germany). Her research is focused on RNA metabolism and RNA-protein interactions, and the evolution of complex RNA enzymes. Eric Westhof is Professor of Biophysics at the Université Louis Pasteur in Strasbourg (France) since 1988. He studied in Liège and Regensburg and was a Fulbright-Hays Research Fellow at the University of Wisconsin with M. Sundaralingam. His research is centered on the relationships between sequence, structure, evolution and functions of RNA molecules.

2

Biochemie, Molekularbiologie

RNA SYNTHESIS Enyzmatic synthesis, ligation and modification Chemical synthesis STRUCTURE DETERMINATION Sequence determination Secondary and tertiary structure determination Footprinting techniques Identification of interacting proteins and nucleic acids Nucleotide analog interference mapping Atomic structure determination Spectroscopic studies of RNA structure GENOMICS & BIOINFORMATICS Phylogenetic analysis Structure prediction Molecular dynamics simulation RNA mining and RNomics ANALYSIS OF RNA FUNCTION Isolation and purification of RNA-binding proteins Genetic analysis of RNA-binding proteins Three-hybrid analysis to identify RNA-binding proteins Analysis of mRNA splice variants Confocal microscopy studies of intracellular localization In vitro selection of ribozymes and aptamers RNA INTERFERENCE Strategies for in vivo RNAi studies (partial contents)

240 mm

17 cm