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Beschreibung
Somuchofwhatweknowaboutthepathogenesisofhumandiseasehascomefromthe systematic and careful study of histological material. Indeed, every internal medicine discipline has its landmark papers describing the clinico-pathological correlations. However, increasingly, it is molecular and cellular biology that provides the necessary mechanistic insights. For many years, it was thought that the two skill… sets were mutually exclusive, but we hope that this book shows that this is not necessarily so. Implicitinthescienceofhistologyisthepreservationandarchivingoftissue.PartIof the book concentrates on the preparation of tissue, providing an overview of fixation, embedding, and processing (Chapter 1), and in Chapters 2 and 3, the required techniques for the retrieval of RNA from histological sections. Both routine and specialist histological staining techniques are provided in Part II. These include pro- cols for immuno (Chapters 4–7), lectin (Chapter 8), and hybridization (Chapter 9) histochemistry,histologicalstaining (Chapters10and11),aswellasspecificmethods for the in situ identification of hypoxia (Chapter 12) and apoptosis (Chapter 13). Finally, Part III details advances in imaging (Chapters 14–16) and image analysis (Chapter 17). It is hoped that this volume will provide molecular biologists with the basic his- chemical techniques and histologists with the molecular techniques to realise the potential of their resource. We are indebted to the authors for their generosity in sharing these protocols. Dieses Produkt haben wir gerade leider nicht auf Lager.
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Bis zu 50 % günstiger als neu
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Technische Daten
Erscheinungsdatum
16.12.2009
Sprache
Englisch
EAN
9781603273442
Herausgeber
Humana Press
Serien- oder Bandtitel
Methods in Molecular Biology
Sonderedition
Nein
Seitenanzahl
229
Einbandart
Gebundene Ausgabe
Einbandart Details
Laminiert
Bandzählung
611
Schlagwörter
Histochemistry, Histological material, Imaging techniques, Laboratory, Pathogenesis, Staining techniques, Tissue processing, electron microscopy, gene expression, genes, hybridization, molecular biology
Thema-Inhalt
PSF - Zellbiologie (Zytologie)
PSB - Biochemie
MBGR - Medizinische Forschung
Höhe
254 mm
Breite
17.8 cm
Transparenz & Sicherheit
Hersteller: Springer Nature B.V., ProductSafety@springernature.com, Springer Nature Customer Service Center GmbH
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