Table of contents
Table of contents. V
List of symbols and abbreviations. VII
1 Introduction. 1
1.1 Motivation. 1
1.2 Structure of this thesis. 4
2 Theoretical background and instrumentation. 7
2.1 Confocal microscopy. 7
2.2 Doughnut-mode laser beams. 11
2.3 Microresonators. 15
2.4 Principles of molecular emission. 24
3 Focusing of doughnut-mode laser beams in freespace
and in a λ/2-microresonator. 27
3.1 Introduction. 28
3.2 Focusing of doughnut-mode laser beams in freespace. 29
3.3 Focusing of doughnut-mode laser beams in a λ/2-microresonator. 35
3.4 Experimental verification of the modelling. 41
3.5 Conclusion. 44
4 Longitudinal localization of a fluorescent bead with an
accuracy of λ/60. 45
4.1 Introduction. 46
4.2 Sample preparation. 47
4.3 Longitudinal localization with a RPDB. 48
4.4 Excitation with an APDB. 51
4.5 Conclusion. 52
Table of contents
5 Three-dimensional orientation of single molecules in
freespace and in a λ/2-microresonator. 53
5.1 Introduction. 54
5.2 Three-dimensional orientation of single molecules in freespace. 55
5.3 Three-dimensional orientation of single molecules in a λ/2-microresonator. 59
5.4 Conclusion. 66
6 Control of the Förster resonance energy transfer
in a tunable λ/2-microresonator. 67
6.1 Introduction. 68
6.2 Sample preparation. 70
6.3 FRET in a tunable λ/2-microresonator. 72
6.4 Conclusion. 74
7 Summary. 75
7.1 Summary of results. 75
7.2 Outlook and future work. 77
7.3 Zusammenfassung. 78
8 References. 81
9 Appendix. 93
9.1 Scientific contributions. 93
9.2 Academic teachers. 97
9.3 Acknowledgement. 98
9.4 Curriculum vitae. 100 http://api.vlb.de/api/v1/asset/mmo/file/0c44649d-36be-4296-a48e-1aa71c916882
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