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Bücher Englische Bücher Wissen & Bildung Naturwissenschaft, Medizin, Informatik, Technik Chemie Theoretische Chemie Fluorescent Tools for Imaging Oxidative Stress in Biology
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Fluorescent Tools for Imaging Oxidative Stress in Biology
Amandeep Kaur (Taschenbuch, Englisch)
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Beschreibung
This thesis advances the long-standing challenge of measuring oxidative stress and deciphering its underlying mechanisms, and also outlines the advantages and limitations of existing design strategies. It presents a range of approaches for the chemical synthesis of fluorescent probes that detect reversible changes in cellular oxidative stress. The ability to visualise cellular processes in… real-time is crucial to understanding disease development and streamline treatment, and this can be achieved using fluorescent tools that can sense reversible disturbances in cellular environments during pathogenesis. The perturbations in cellular redox state are of particular current interest in medical research, since oxidative stress is implicated in the pathogenesis of a number of diseases. The book investigates different strategies used to achieve ratiometric fluorescence output of the reversible redox probes, which nullify concentration effects associated with intensity-based probes. It also describes suitable approaches to target these probes to specific cellular organelles, thereby enabling medical researchers to visualise sub-cellular oxidative stress levels, and addressing the typically poor uptake of chemical tools into biological studies. In total it reports on four new probes that are now being used by over twenty research groups around the globe, and two of which have been commercialised. The final chapters of this thesis demonstrate successful applications of the sensors in a variety of biological systems ranging from prokaryotes to mammalian cells and whole organisms. The results described clearly indicate the immense value of collaborative, cross-disciplinary research. Dieses Produkt haben wir gerade leider nicht auf Lager.
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Technische Daten
Erscheinungsdatum
22.12.2018
Sprache
Englisch
EAN
9783030103712
Herausgeber
Springer International Publishing
Serien- oder Bandtitel
Springer Theses
Sonderedition
Nein
Autor
Amandeep Kaur
Seitenanzahl
230
Auflage
Softcover reprint of the original 1st edition 2018
Einbandart
Taschenbuch
Schlagwörter
Ratiometric Fluorescent Probes, Oxidative Stress, Flavin-based Redox Probes, Nicotinamide-based Redox Probes, FRET-based Redox Probes, Biological Imaging, Cellular Redox State, Embryonic Stem Cells, Pancreatic Cancer Cells, Ex Vivo Studies
Thema-Inhalt
PNF - Analytische Chemie
PSF - Zellbiologie (Zytologie)
PHVN - Biophysik
TCB - Biotechnologie
PNR - Physikalische Chemie
Höhe
235 mm
Breite
15.5 cm
Hersteller: Springer, Europaplatz 3, Heidelberg, Deutschland, 69115, ProductSafety@springernature.com, Springer Nature Customer Service Center GmbH
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