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Beschreibung
Mitosis: Methods and Protocols provides state-of-the-art overviews on the most important approaches currently used in mitosis research spanning from the analysis of single molecules in isolation to their utilization within the complex environment of the cell. The volume is divided into four parts, each focused on methods pertaining to distinct aspects of mitosis research. Part I presents… approaches for visualizing and analyzing the dynamic behaviors of the spindle apparatus, the microtubule based machine that drives chromosome segregation. Part II focuses more generally on methods for studying and manipulating the microtubule cytoskeleton in cells and complex cell free extracts. Part III provides state of the art biophysical and high resolution microscopy approaches for assessing complex interactions between microtubules and microtubule-associated proteins in isolation as well as microtubule structure in cells. Part IV provides methods for studying the effects of cell shape on cell division and methods for quantifying aneuploidy (aberrant chromosome number) which frequently results from mitotic defects and has been linked to human maladies ranging from birth defects to cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mitosis: Methods and Protocols seeks to provide diverse methods and new techniques to address new or old questions related to the mechanisms of mitosis. Dieses Produkt haben wir gerade leider nicht auf Lager.
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Technische Daten
Erscheinungsdatum
15.03.2014
Sprache
Englisch
EAN
9781493903283
Herausgeber
Springer US
Serien- oder Bandtitel
Methods in Molecular Biology
Sonderedition
Nein
Seitenanzahl
308
Einbandart
Gebundene Ausgabe
Buch Untertitel
Methods and Protocols
Schlagwörter
cell division, chromosome segregation, complex cell free extracts, microtubule based machine, microtubule cytoskeleton in cells, microtubule structure in cells, microtubule-associated proteins, microtubules, mitosis, mitotic defects, quantifying aneuploidy, spindle apparatus, Cell Cycle Analysis, gene expression
Thema-Inhalt
PSF - Zellbiologie (Zytologie)
PDN - Wissenschaftliche Ausstattung, Experimente und Techniken
PSAK - Genetik (nicht-medizinisch)
Höhe
254 mm
Breite
17.8 cm
Hersteller: Springer Nature Customer Service Center GmbH, Europaplatz 3, Heidelberg, Deutschland, 69115, ProductSafety@springernature.com
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