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Bücher Englische Bücher Wissen & Bildung Naturwissenschaft, Medizin, Informatik, Technik Biologie Mikrobiologie Invertebrate Tissue Culture Methods
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Invertebrate Tissue Culture Methods
Jun Mitsuhashi (Broschiert, Englisch)
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Beschreibung
I started insect cell culture work in 1962, when T. D. C. Grace reported the first establishment of invertebrate continuous cell lines. He obtained grow ing cells from pupal ovaries of the emperor gum moth, Antheraea euca lypti. At that time, I was trying to obtain growing cells from leafhoppers. Grace's method could not be applied directly to my culture because of the differences in species, the… size of the insects, and the tissue to be cul tured. The vertebrate tissue culture methods gave me some ideas for pre paring cultures from leafhoppers, but those could not be used directly either. There were no textbooks and no manuals for invertebrate tissue culture, so I had to develop a method by myself. First, I considered what type and what size of vessels are suitable for insect tissue culture. Also, I had to look for suitable materials to construct the culture vessels. Sec ond, I had to examine various culture media, especially growth-promot ing substances, such as sera. Then I had to improve culture media by trial and error. The procedure to set up a primary culture was also a problem. How could I sterilize materials? How could I remove tissues from a tiny insect? How many tissues should I pool in order to set up one culture? I had to find out the answers. Naturally, it took a lot of time. 139,09 €
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Technische Daten
Erscheinungsdatum
01.02.2002
Sprache
Englisch
EAN
9784431703136
Herausgeber
Springer Tokyo
Serien- oder Bandtitel
Springer Lab Manuals
Sonderedition
Nein
Autor
Jun Mitsuhashi
Seitenanzahl
446
Einbandart
Broschiert
Schlagwörter
Annelida, Chordata, Echinodermata, Insecta, Mollusca, Orthoptera, Plathelminthes, Porifera, invertebrates, tissue
Thema-Inhalt
PSF - Zellbiologie (Zytologie)
PSV - Zoologie und Tierwissenschaften
Höhe
235 mm
Breite
15.5 cm
Transparenz & Sicherheit
Hersteller: Springer, Europaplatz 3, Heidelberg, Deutschland, 69115, ProductSafety@springernature.com, Springer Nature Customer Service Center GmbH
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