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Beschreibung
The manual consists of two main sections. The first includes the essential, sometimes laborious, procedures for handling yeasts, for inducing mating and isolation of hybrids, for inducing sporulation and isolation of single-spore clones, with some details of tetrad analysis, and including techniques and ancillary equipment for use of the micromanipulator. There are also proce dures for induction… of mutants by physical and chemical agents, and for iso lation of particular types of mutants, such as to temperature sensitivity, for increased frequency of mutations, for mutations in the mitochondrial genome, both to the petite colonie form and to resistance to antibiotics, for mutations in that part of the yeast genome controlling the glycolytic cycle, and numerous others. Mapping of mutations is discussed briefly, though this aspect of yeast genetics is probably one which should not be undertaken until the investigator has gained a certain amount of experience in the field. How ever, as is pointed out in the pertinent part of the manual, the task of mapping has been tremendouslysimplified by the availability from the Yeast Genetics Stock Center at the University of California at Berkeley of a set of auxo trophic strains designed to permit mapping of most unknown genes with a minimum number of crosses and tetrad analyses. The first section concludes with the description of methods for hybridization of yeasts by protoplast fusion, which has been described as the poor man's system for genetic engineering. 106,99 €
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Technische Daten
Erscheinungsdatum
23.12.1988
Sprache
Englisch
EAN
9783540188056
Herausgeber
Springer Berlin
Sonderedition
Nein
Autor
John F.T. Spencer, Dorothy M. Spencer, I.J. Bruce
Seitenanzahl
104
Auflage
1
Einbandart
Broschiert
Buch Untertitel
A Manual of Methods
Schlagwörter
DNA, RNA, chromosome, enzyme, enzymes, genetics, mutagenesis, mutation, protoplast, yeast
Thema-Inhalt
TCB - Biotechnologie
PSF - Zellbiologie (Zytologie)
PSB - Biochemie
Höhe
235 mm
Breite
15.5 cm
Transparenz & Sicherheit
Hersteller: Springer, Europaplatz 3, Heidelberg, Deutschland, 69115, ProductSafety@springernature.com, Springer Nature Customer Service Center GmbH
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