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Bücher Englische Bücher Wissen & Bildung Naturwissenschaft, Medizin, Informatik, Technik Biologie Mikrobiologie Microinjection and Transgenesis
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Beschreibung
The establishment of microinjection protocols about 20 years ago for cultured cells and shortly thereafter for the generation of transgenic mice by microinjection of DNA into fertilized mouse eggs greatly influ enced many fields of biology. Not only have the data generated using these approaches contributed to a large extent to our present under standing of gene regulation and cellular function of… higher eukaryotic cells, but current knowledge and future developments in this area will certainly have a great impact on basic and applied research for many years to come. This laboratory manual describes the current state of the art in this research area and focuses primarily on both the experimental strategies with an extensive bibliography and the detailed procedures. A large number of studies are presently being performed and a great variety of new experimental designs are rapidly being developed. The book con tains protocols on injection of somatic cells as well as on injection of embryos, the use of similar equipment being a common feature. In the articles dedicated to somatic cells, full descriptions of the manual and automatic injection systems are given as well as the methods for the analysis of injected cells by video-microscopy, electron microscopy or in situ hybridizations. In addition, comprehensive protocols are given for injection experiments with very different purposes, such as to study sig nal transduction or microtubule dynamics. 53,49 €
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Technische Daten
Erscheinungsdatum
27.11.1997
Sprache
Englisch
EAN
9783540618959
Herausgeber
Springer Berlin
Serien- oder Bandtitel
Springer Lab Manuals
Sonderedition
Nein
Seitenanzahl
619
Auflage
1
Einbandart
Broschiert
Buch Untertitel
Strategies and Protocols
Schlagwörter
Vivo, biochemistry, cell, cell culture, chromosome, electron microscopy, gene, gene expression, gene transfer, microscopy, proteins, ribozyme, stem cells, tissue, transcription
Thema-Inhalt
PSF - Zellbiologie (Zytologie)
Höhe
242 mm
Breite
19.3 cm
Transparenz & Sicherheit
Hersteller: Springer, Europaplatz 3, Heidelberg, Deutschland, 69115, ProductSafety@springernature.com, Springer Nature Customer Service Center GmbH
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