iPhoneAlle anzeigen
iPhone 11iPhone 11 ProiPhone 11 Pro MaxiPhone 12iPhone 12 ProiPhone 12 Pro MaxiPhone 12 miniiPhone 13iPhone 13 ProiPhone 13 Pro MaxiPhone 13 miniiPhone 14iPhone 14 PlusiPhone 14 ProiPhone 14 Pro MaxiPhone 15iPhone 15 PlusiPhone 15 ProiPhone 15 Pro MaxiPhone 16iPhone 16 PlusiPhone 16 ProiPhone 16 Pro MaxiPhone 16eiPhone 8iPhone 8 PlusiPhone SE 2020iPhone SE 2022iPhone XiPhone XRiPhone XSiPhone XS Max
Galaxy A-SerieAlle anzeigen
Galaxy A12Galaxy A13Galaxy A13 5GGalaxy A14Galaxy A15Galaxy A16Galaxy A17Galaxy A17 5GGalaxy A20eGalaxy A21Galaxy A22Galaxy A23Galaxy A24Galaxy A25Galaxy A26 5GGalaxy A32Galaxy A33Galaxy A34Galaxy A35Galaxy A36 5GGalaxy A37Galaxy A40Galaxy A41Galaxy A42Galaxy A50Galaxy A51Galaxy A52Galaxy A53Galaxy A54Galaxy A55Galaxy A56 5GGalaxy A57 5GGalaxy A70Galaxy A71Galaxy A72
Redmi SeriesAlle anzeigen
Redmi 10Redmi 13CRedmi 15 5GRedmi Note 10Redmi Note 10 ProRedmi Note 11Redmi Note 11 ProRedmi Note 11 Pro Plus 5GRedmi Note 11sRedmi Note 12Redmi Note 12 5GRedmi Note 12 ProRedmi Note 12 Pro 5GRedmi Note 12 Pro PlusRedmi Note 13Redmi Note 13 5GRedmi Note 13 ProRedmi Note 13 Pro PlusRedmi Note 14Redmi Note 14 Pro PlusRedmi Note 15Redmi Note 15 5GRedmi Note 15 ProRedmi Note 15 Pro 5GRedmi Note 15 Pro Plus 5GRedmi Note 8Redmi Note 8 ProRedmi Note 9Redmi Note 9 Pro
- Startseite
Bücher Englische Bücher Wissen & Bildung Naturwissenschaft, Medizin, Informatik, Technik Medizin Veterinärmedizin Minimalistic immunologically defined gene expression T helper cell 1 (MIDGE-Th1®) vectors coding for Interleukin 12 and -18 in combination with the transfection agent SAINT-18 have systemic antitumoral effects on equine melanomas
Handgeprüfte Gebrauchtware
Bis zu 50 % günstiger als neu
Der Umwelt zuliebe
Minimalistic immunologically defined gene expression T helper cell 1 (MIDGE-Th1®) vectors coding for Interleukin 12 and -18 in combination with the transfection agent SAINT-18 have systemic antitumoral effects on equine melanomas
Kathrin Mählmann (Unbekannter Einband, Englisch)
★★★★★
☆☆☆☆☆ Keine Bewertungen vorhanden
Optischer Zustand
Beschreibung
Equine melanomas are the most common neoplasms in older grey horses. Until now, no curative therapy is established. Xenogenic DNA vaccination represents a promising therapeutic approach.
Goal of the present study was to evaluate the safety and clinical efficacy of vaccination of grey horses with natural occurring melanomas with MIDGE-Th1® vectors encoding for equine (eq) IL12 and eqILRAP-IL18… alone or in combination with human gp100 (hgp100) or human tyrosinase (htyr).
Three groups (each n=9) of adult grey horses with variable numbers of melanomas received three intramuscular and three intradermal peritumoral vaccinations. Each group was treated with MIDGE-Th1® vectors coding for eqIL12 and eqILRAP-IL18. One group (group gp100) was additionally treated with 500 μg hgp100MIDGE-Th1® and one group with 500 μg htyrMIDGE-Th1® (group tyr). The third group did not receive additional DNA (group IL12/18). Vectors were complexed with the transfection agent SAINT-18.The injections were performed on days 1, 22 and 78 intradermally around one selected melanoma (locally treated melanoma) and intramuscularly as a systemic treatment. A general examination of the horses and evaluation of local reactions at the injection site were performed daily during hospitalisation (on the day of each injection and 3 days after each injection as well as on day 120). In each horse the locally treated melanoma and up to 8 non-locally treated melanomas were measured before each injection and on day 120 by calliper and ultrasound. The relative volumes were calculated in relation to day 1 (100%).
For the evaluation of the generation of specific CD8+ cytotoxic T-cells against hgp100 and htyr, peripheral blood mononuclear cells (PBMCs) isolated before each injection and on day 120, were stimulated in vitro with autologous dermal cells transfected with hgp100 or htyr. Interferon γ (IFN γ) as a response to this specific stimulation was measured by intracellular immunofluorescence staining.
Specific serum antibodies against hgp100 and htyr were measured at equivalent time points as cytotoxic T-cells using a cell based flow cytometric assay. Human embryonic kidney cells, transfected with hgp100 or htyr served as a source for antigens.
The only consistent abnormal finding upon clinical examination of the treated horses was an increased body temperature. A significant increase in body temperature (38.7 ± 0.59 °C) was recorded after 40 of the 81 injections, and usually lasted for one day. Local inflammatory reactions and depigmentation were observed in all groups starting at day 3 after the first application.
The relative tumor volume (%) was significantly reduced to 71.5 ± 29.73% (calliper measurements) and 87.0 ± 24.42% (ultrasound measurements). There were no significant differences detected between groups (gp100, tyr and IL12/18). No difference was seen in reduction of tumor volume between locally treated and non-locally treated melanoma.
Neither a cellular nor a humoral immune response against the vaccine antigens could be detected.
In this study, treatment with eqIL12 and eqILRAP-IL18 DNA in combination with SAINT-18 showed a systemic effect against grey horse melanoma. There was no additional clinical or immunological effect observed by vaccination with MIDGE-Th1® vectors encoding hgp100 and htyr. It remains unclear whether the antitumoral effect was caused by the interleukins, unmethylated CpG motifs or the induction of an immune reaction by the combination of DNA and transfection reagent.
Reasons for the absence of a specific immune response may be an inadequate selection of dosages or treatment interval. It is also possible that the sensitivity of the immunoassays was too low to detect a subclinical immune response.
Further studies to characterize the antitumoral effects of the treatment used in this study and modifications of the treatment protocol to achieve a potential specific immune response are warranted. 30,30 €
Unbekannter Einband | Neu
oder
zzgl.
Du kannst wie immer einen Kaufalarm setzen, wenn du auf das gebrauchte Buch warten möchtest.
zzgl.
Handgeprüfte Gebrauchtware
Bis zu 50 % günstiger als neu
Der Umwelt zuliebe
Technische Daten
Erscheinungsdatum
23.11.2012
Sprache
Englisch
EAN
9783954042753
Herausgeber
Cuvillier Verlag
Serien- oder Bandtitel
Wissenschaftliche Reihe der Klinik für Pferde
Sonderedition
Nein
Autor
Kathrin Mählmann
Seitenanzahl
192
Auflage
1
Einbandart
Unbekannter Einband
Bandzählung
3
Schlagwörter
Spezielle Veterinärmedizin, Equine, Xengenic, Melanoma, DNA Vaccination, SAINT-18, MIDGE
Höhe
2100 mm
Breite
148 cm
Transparenz & Sicherheit
Hersteller: Cuvillier, Nonnenstieg 8, Göttingen, Deutschland, 37075, info@cuvillier.de, info@cuvillier.de
-.-
★★★★★
☆☆☆☆☆ Leider noch keine Bewertungen
Leider noch keine Bewertungen
Sicher bei rebuy kaufen
Schreib die erste Bewertung für dieses Produkt!
Wenn du eine Bewertung für dieses Produkt schreibst, hilfst du allen Kund:innen, die noch überlegen, ob sie das Produkt kaufen wollen. Vielen Dank, dass du mitmachst!
Sicher bei rebuy kaufen