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Bücher Wissen & Bildung Englische Bücher Naturwissenschaft, Medizin, Informatik, Technik Biologie Mikrobiologie PCR Sequencing Protocols
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Beschreibung
Advances in bioscience research usually arise as a result of the continu ing refinement of existing technologies. However, there are a number of occa sions v^rhere newly developed methodologies have a profound effect on nearly all areas of research. Frequently these are techniques that are elegantly simple in concept and require minimal technical manipulation. Two of these revolu tionary… techniques are the focus ofPCR Sequencing Protocols. The first such technique is enzymatic chain termination sequencing developed by Sanger and his co-workers in Cambridge and reported in 1977. This essentially brought the possibility of deriving nucleotide sequence information in a very short time scale and has been widely accepted in many laboratories as a routine molecular biological research tool. Furthermore, it has not only led to the sequencing of many genes and gene fragments, but has also allowed the tech nical means of sequencing the human genome. The second technique that has found widespread acceptance in basic applied research and many routine applications is the polymerase chain reac tion. This technique, first reported in 1985 by MuUis and his colleagues, pro vides the means to amplify nucleic acid sequence, which immediately proved invaluable in nearly all fields of biological laboratory research. Here, as with enzymatic DNA sequencing, is a very simple concept that relies on minimal information to prepare short oligonucleotide primers that direct the synthesis of a specified fi-agment o f DNA in the presence of a thermostable DNA polymerase. Dieses Produkt haben wir gerade leider nicht auf Lager.
Handgeprüfte Gebrauchtware
Bis zu 50 % günstiger als neu
Der Umwelt zuliebe
Technische Daten
Erscheinungsdatum
19.08.1996
Sprache
Englisch
EAN
9780896033443
Herausgeber
Humana Press
Serien- oder Bandtitel
Methods in Molecular Biology
Sonderedition
Nein
Seitenanzahl
221
Einbandart
Spiralbindung
Bandzählung
65
Thema-Inhalt
PSF - Zellbiologie (Zytologie)
Höhe
229 mm
Breite
15.2 cm
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