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Bücher Englische Bücher Wissen & Bildung Naturwissenschaft, Medizin, Informatik, Technik Biologie Biochemie & Biophysik Infection dynamics and virus-induced apoptosis in influenza virus A infected adherent and suspension MDCK cells
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Infection dynamics and virus-induced apoptosis in influenza virus A infected adherent and suspension MDCK cells
Britta Peschel (Broschiert, Englisch)
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Beschreibung
Besides the classical production in embryonated chicken eggs, influenza vaccines can also be produced in cell culture-based processes. In order to contribute to the optimisation of these processes, aim of this thesis was to characterise influenza virus infection dynamics and virus-induced apoptosis of host cells. Flow cytometric staining of the influenza virus NP protein was used to monitor the… time course of infection and a TUNEL assay to characterise progress of apoptosis.
Firstly, infections of adherent Madin-Darby canine kidney (MDCK) cells with different influenza virus strains at several multiplicity of infection (MOI) were performed, followed by studies on defective interfering particles (DIPs). Secondly, infections of adherent MDCK cells were additionally performed in stirred tank bioreactors. Finally, the MDCK.SUS2 suspension cell line was characterised for influenza virus propagation.
In summary, this work demonstrated low MOI infection conditions to be beneficial for optimisation of infectious virus yields in influenza A virus production. Infection dynamics depended, however, on virus strain, especially in terms of virus-induced apoptosis. In addition, presence of DIPs in seed virus strongly influenced infectious virus yields. Finally, a MDCK suspension cell line was demonstrated to be an alternative to adherent MDCK cells, offering a production process that is easier to scale-up. Thereby, this work contributed to the understanding of influenza virus propagation in cell culture, and provided valuable insights for optimisation of upstream processing in influenza vaccine production. Dieses Produkt haben wir gerade leider nicht auf Lager.
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Technische Daten
Erscheinungsdatum
01.07.2015
Sprache
Englisch
EAN
9783844037388
Herausgeber
Shaker
Serien- oder Bandtitel
Forschungsberichte aus dem Max-Planck-Institut für Dynamik komplexer technischer Systeme
Sonderedition
Nein
Autor
Britta Peschel
Seitenanzahl
194
Auflage
1
Einbandart
Broschiert
-.-
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